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anti trf2 mouse monoclonal  (Novus Biologicals)


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    Structured Review

    Novus Biologicals anti trf2 mouse monoclonal
    Anti Trf2 Mouse Monoclonal, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+monoclonal+anti+trf2/TRF-2+Antibody+(4A794%2E15)+-+Azide+Free/pm36426578-439-7-10
    Average 93 stars, based on 3 article reviews
    anti trf2 mouse monoclonal - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: Protein requirements for sister telomere association in human cells
    Article Snippet: .. Blots were incubated with the following primary antibodies: goat anti-SA1 BL140G (1 μg/ml) (Bethyl Laboratories Inc.); goat anti-SA1 BL143G (1 μg/ml) (Bethyl Laboratories Inc.); goat anti-SA2 BL146G (1 μg/ml) (Bethyl Laboratories Inc.); rabbit anti-Scc1 (2 mg/ml, Bethyl Laboratories Inc.); rabbit anti-Smc3 (0.2 mg/ml) (Calbiochem); rabbit anti-TRF1 415 (1 μg/ml) ( Cook et al , 2002 ); rabbit anti-tankyrase1 609 (1 μg/ml)( Cook et al , 2002 ); mouse monoclonal anti-TRF2 (2.0 μg/ml) (Imgenex), rabbit anti-Myc (0.8 μg/ml) (Santa Cruz Biotechnologies); mouse monoclonal anti-Flag M2 (4.3 μg/ml) (Sigma); rabbit anti-TIN2 701 (0.5 μg/ml) ( Houghtaling et al , 2004 ); mouse anti-a-tubulin ascites (1:50 000) (Sigma); or rabbit anti-GFP serum (1:2500) (Abcam), followed by horseradish peroxidase-conjugated donkey anti-rabbit (Amersham), anti-mouse (Amersham), or anti-goat IgG (Bethyl Laboratories Inc.) (1:2500). .. Bound antibody was detected with Super Signal West Pico (Pierce).

    Article Title: The TEL patch of telomere protein TPP1 mediates telomerase recruitment and processivity
    Article Snippet: .. Cells were incubated with mouse monoclonal anti-TRF2 (Imgenex; IMG-124A; 1:500) and rabbit polyclonal anti-53BP1 (Novus Biologicals; NB100–304; 1:1000 dilution) in PBS-T containing nuclease-free 3% BSA for 1 h. The cells were then washed three times in PBS (5 min each), and incubated with Alexa Fluor 568-conjugated anti-mouse IgG (Life Technologies) and Alexa Fluor 647-conjugated anti-rabbit IgG (Life Technologies) diluted 1:500 in PBS-T containing nuclease-free 3% BSA for 30 min in the dark. ..

    Article Title: TRF1 is degraded by ubiquitin-mediated proteolysis after release from telomeres
    Article Snippet: .. TRAP assays ( Kim et al. 1994 ) contained 1 μg CHAPS (Pierce) extract with or without 10 μg/mL RNase A. Immunoblotting Immunoblots were incubated with the following primary antibodies: rabbit anti-poly(ADP-ribose) serum (1:1000; Alexis Biochemicals), rabbit anti-TRF1 415 (0.2 μg/mL; Cook et al. 2002 ), rabbit anti-tankyrase 1 376 (0.1 μg/mL; Cook et al. 2002 ), mouse anti-α-tubulin ascites (1:500,000; Sigma), rabbit anti-TERT 374 (0.8 μg/mL; raised and affinity purified against Escherichia coli -derived fusion protein containing hTERT amino acids 561–698), or mouse monoclonal anti-TRF2 (1.0 μg/mL; Imgenex Clone 4A794), followed by horseradish peroxidase-conjugated donkey anti-rabbit or anti-mouse IgG (Amersham; 1:2500). .. Bound antibody was detected using the Enhanced Chemiluminescence (Amersham), Super-Signal West Dura, or Femto (Pierce) kits.

    Article Title: Image-aided Suicide Gene Therapy Utilizing Multifunctional hTERT-targeting Adenovirus for Clinical Translation in Hepatocellular Carcinoma
    Article Snippet: .. Cells on the coverslip were incubated with specific primary antibody, mouse monoclonal anti-TRF2 (IMGENEX, San Diego, CA, USA) and rabbit polyclonal anti-53BP1, anti-γ-H2AX (Bethyl Laboratories, Inc., Montgomery, AL, USA) after permeabilization and blocking, washed, and secondary antibody added. .. For the telomere ends FISH, cells were fixed again, dehydrated, and incubated with denatured telomere probe (Cy3-oo-(TTAGGG) 3 PNA, Panagene, Daejeon, Korea) in 70% formamide in 2× SSC, 0.25% nucleotide blocking reagent, 5% MgCl 2 at 90°C, washing, and 4'-6-diamidino-2-phenylindole (DAPI) staining.

    Article Title: Inhibition of Telomerase Recruitment and Cancer Cell Death
    Article Snippet: .. At 60% confluency, cells were fixed in PBS containing 4% formaldehyde for 10 min, washed with PBS, permeabilized in PBS containing 0.5% Triton X-100 for 5 min, and incubated with primary antibodies (mouse monoclonal anti-TRF2 (Imgenex, catalog no. IMG-124A, 1:500) and rabbit polyclonal anti-53BP1 (Novus Biologicals, catalog no. NB100-304, 1:1000)) for 1 h. After removal of the primary antibodies and three wash steps, cells were incubated with secondary antibodies (Alexa Fluor 568-conjugated antimouse IgG (Invitrogen, 1:500) and Alexa Fluor 647-conjugated anti-rabbit IgG (Invitrogen, 1:500)) for 30min in the dark. .. After removal of the secondary antibodies and three subsequentwash steps, the coverslips were mounted on microscope slides using Vectashield mounting medium with DAPI (Vector Laboratories), sealed with transparent nail polish, and stored in the dark at 20 °C until imaging was performed.

    Western Blot:

    Article Title: TRF1 is degraded by ubiquitin-mediated proteolysis after release from telomeres
    Article Snippet: .. TRAP assays ( Kim et al. 1994 ) contained 1 μg CHAPS (Pierce) extract with or without 10 μg/mL RNase A. Immunoblotting Immunoblots were incubated with the following primary antibodies: rabbit anti-poly(ADP-ribose) serum (1:1000; Alexis Biochemicals), rabbit anti-TRF1 415 (0.2 μg/mL; Cook et al. 2002 ), rabbit anti-tankyrase 1 376 (0.1 μg/mL; Cook et al. 2002 ), mouse anti-α-tubulin ascites (1:500,000; Sigma), rabbit anti-TERT 374 (0.8 μg/mL; raised and affinity purified against Escherichia coli -derived fusion protein containing hTERT amino acids 561–698), or mouse monoclonal anti-TRF2 (1.0 μg/mL; Imgenex Clone 4A794), followed by horseradish peroxidase-conjugated donkey anti-rabbit or anti-mouse IgG (Amersham; 1:2500). .. Bound antibody was detected using the Enhanced Chemiluminescence (Amersham), Super-Signal West Dura, or Femto (Pierce) kits.

    Affinity Purification:

    Article Title: TRF1 is degraded by ubiquitin-mediated proteolysis after release from telomeres
    Article Snippet: .. TRAP assays ( Kim et al. 1994 ) contained 1 μg CHAPS (Pierce) extract with or without 10 μg/mL RNase A. Immunoblotting Immunoblots were incubated with the following primary antibodies: rabbit anti-poly(ADP-ribose) serum (1:1000; Alexis Biochemicals), rabbit anti-TRF1 415 (0.2 μg/mL; Cook et al. 2002 ), rabbit anti-tankyrase 1 376 (0.1 μg/mL; Cook et al. 2002 ), mouse anti-α-tubulin ascites (1:500,000; Sigma), rabbit anti-TERT 374 (0.8 μg/mL; raised and affinity purified against Escherichia coli -derived fusion protein containing hTERT amino acids 561–698), or mouse monoclonal anti-TRF2 (1.0 μg/mL; Imgenex Clone 4A794), followed by horseradish peroxidase-conjugated donkey anti-rabbit or anti-mouse IgG (Amersham; 1:2500). .. Bound antibody was detected using the Enhanced Chemiluminescence (Amersham), Super-Signal West Dura, or Femto (Pierce) kits.

    Derivative Assay:

    Article Title: TRF1 is degraded by ubiquitin-mediated proteolysis after release from telomeres
    Article Snippet: .. TRAP assays ( Kim et al. 1994 ) contained 1 μg CHAPS (Pierce) extract with or without 10 μg/mL RNase A. Immunoblotting Immunoblots were incubated with the following primary antibodies: rabbit anti-poly(ADP-ribose) serum (1:1000; Alexis Biochemicals), rabbit anti-TRF1 415 (0.2 μg/mL; Cook et al. 2002 ), rabbit anti-tankyrase 1 376 (0.1 μg/mL; Cook et al. 2002 ), mouse anti-α-tubulin ascites (1:500,000; Sigma), rabbit anti-TERT 374 (0.8 μg/mL; raised and affinity purified against Escherichia coli -derived fusion protein containing hTERT amino acids 561–698), or mouse monoclonal anti-TRF2 (1.0 μg/mL; Imgenex Clone 4A794), followed by horseradish peroxidase-conjugated donkey anti-rabbit or anti-mouse IgG (Amersham; 1:2500). .. Bound antibody was detected using the Enhanced Chemiluminescence (Amersham), Super-Signal West Dura, or Femto (Pierce) kits.

    Blocking Assay:

    Article Title: Image-aided Suicide Gene Therapy Utilizing Multifunctional hTERT-targeting Adenovirus for Clinical Translation in Hepatocellular Carcinoma
    Article Snippet: .. Cells on the coverslip were incubated with specific primary antibody, mouse monoclonal anti-TRF2 (IMGENEX, San Diego, CA, USA) and rabbit polyclonal anti-53BP1, anti-γ-H2AX (Bethyl Laboratories, Inc., Montgomery, AL, USA) after permeabilization and blocking, washed, and secondary antibody added. .. For the telomere ends FISH, cells were fixed again, dehydrated, and incubated with denatured telomere probe (Cy3-oo-(TTAGGG) 3 PNA, Panagene, Daejeon, Korea) in 70% formamide in 2× SSC, 0.25% nucleotide blocking reagent, 5% MgCl 2 at 90°C, washing, and 4'-6-diamidino-2-phenylindole (DAPI) staining.



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    Image Search Results


    a The diagram shows the workflow on a timeline: on the day after cell seeding, cells were treated with RHPS4 (0.2 and/or 0.5 μM). At 48 h after treatment, cells were again treated with RHPS4 (0.2 and/or 0.5 μM) or harvested and counted. At 72 h after the first treatment, the cell viability was checked, and finally at 96 h after the first treatment, the cells were harvested and processed. b Representative images showing U251MG cells stained by immunofluorescence using anti-BLM (red signals), and anti-TRF1 or anti-TRF2 (green signals) antibodies. Merged images allow visualization of colocalizing dots (yellow signals). Yellow arrows indicate BLM and TRF1-TRF2 colocalizations. c Quantification of the colocalizations between BLM and TRF1 or TRF2 proteins in U251MG cell line upon RHPS4 treatment (0.2 and 0.5 μM). d , e Sensitivity of U251MG and BLM −/− cell lines to RHPS4 concentrations ranging from 0.01 to 2 μM (0.01; 0.125; 0.25; 0.5; 1; 2 μM), evaluated 96 h after the first treatment. Mitomycin C (MMC) was used as a positive control at concentrations of 0.1; 0.5; 1; 2; 5 μg/ml. The Sulforhodamine B (SRB) cytotoxicity assay showed that RHPS4 sensitivity was unchanged in U251MG and BLM −/− (IC50 was 0.56 μM in both cell lines). f Short-term cell proliferation in untreated cells, U251MG and BLM −/− , and in RHPS4-treated cells (0.5 μM) as evaluated 48 and 96 h after the first treatment. g Long-term cell proliferation in U251MG and BLM −/− untreated and RHPS4-treated cells (0.5 μM). Scale bars represent 5 μm. * p < 0.05, ** p < 0.01 (two-way ANOVA; n = 3). Error bars denote the standard deviation of the mean.

    Journal: Communications Biology

    Article Title: BLM and FANCJ role in the response to G-quadruplex-dependent telomeric replicative stress

    doi: 10.1038/s42003-025-09367-z

    Figure Lengend Snippet: a The diagram shows the workflow on a timeline: on the day after cell seeding, cells were treated with RHPS4 (0.2 and/or 0.5 μM). At 48 h after treatment, cells were again treated with RHPS4 (0.2 and/or 0.5 μM) or harvested and counted. At 72 h after the first treatment, the cell viability was checked, and finally at 96 h after the first treatment, the cells were harvested and processed. b Representative images showing U251MG cells stained by immunofluorescence using anti-BLM (red signals), and anti-TRF1 or anti-TRF2 (green signals) antibodies. Merged images allow visualization of colocalizing dots (yellow signals). Yellow arrows indicate BLM and TRF1-TRF2 colocalizations. c Quantification of the colocalizations between BLM and TRF1 or TRF2 proteins in U251MG cell line upon RHPS4 treatment (0.2 and 0.5 μM). d , e Sensitivity of U251MG and BLM −/− cell lines to RHPS4 concentrations ranging from 0.01 to 2 μM (0.01; 0.125; 0.25; 0.5; 1; 2 μM), evaluated 96 h after the first treatment. Mitomycin C (MMC) was used as a positive control at concentrations of 0.1; 0.5; 1; 2; 5 μg/ml. The Sulforhodamine B (SRB) cytotoxicity assay showed that RHPS4 sensitivity was unchanged in U251MG and BLM −/− (IC50 was 0.56 μM in both cell lines). f Short-term cell proliferation in untreated cells, U251MG and BLM −/− , and in RHPS4-treated cells (0.5 μM) as evaluated 48 and 96 h after the first treatment. g Long-term cell proliferation in U251MG and BLM −/− untreated and RHPS4-treated cells (0.5 μM). Scale bars represent 5 μm. * p < 0.05, ** p < 0.01 (two-way ANOVA; n = 3). Error bars denote the standard deviation of the mean.

    Article Snippet: The slides were then incubated in blocking buffer (1% BSA dissolved in 1X PBS [w/v]) and then incubated overnight (ON) at 4 °C with the primary antibodies (rabbit polyclonal anti-BLM (#A300-110A, Bethyl) (1:100); mouse monoclonal anti-TRF1 (4E4 clone, GTX70304, GeneTex) (1:20) or mouse monoclonal anti-TRF2 (9F10 clone, sc-47693, Santa Cruz Biotechnology) (1:100)).

    Techniques: Staining, Immunofluorescence, Positive Control, Cytotoxicity Assay, Standard Deviation

    Journal: iScience

    Article Title: Human SKI component SKIV2L regulates telomeric DNA-RNA hybrids and prevents telomere fragility

    doi: 10.1016/j.isci.2024.111096

    Figure Lengend Snippet:

    Article Snippet: Mouse monoclonal anti-TRF2 (Clone 4A794) , Millipore , Cat#05-521; RRID: AB_2303145.

    Techniques: Virus, Recombinant, Protease Inhibitor, Reverse Transcription, Blocking Assay, Mass Spectrometry, SYBR Green Assay, Flow Cytometry, Imaging, Mutagenesis, Cell Cycle Assay, shRNA, Software